Journal: Molecular Therapy Oncology

Article Title: Suppression of PARP1 enhances PTEN mRNA therapy in castration-resistant prostate cancer by glycolysis disruption

doi: 10.1016/j.omton.2026.201133

Figure Lengend Snippet: The antitumor effects of mPsiP@miLAND on PC-3 subcutaneous tumor model (A) Brief description of the experimental process and grouping information. (B) Tumor growth curves and (C) bodyweight changes of the treated animals during the entire study period. (D) Tumor weights, (E) serum biochemical indicators, and (F) relative mRNA expression of PARP1, recorded or analyzed at the end of the study. Seven indicators including creatine kinase (CK, U/liter), aspartate aminotransferase (AST, U/liter), alkaline phosphatase (ALP, U/liter), total protein (TP, g/liter), urea nitrogen (UREA, mmol/L), serum creatinine (CREA, mmol/L), and triglyceride (TG, mmol/L) were recorded. (G) Analysis of PI3K-Akt signaling, glycolysis, apoptosis, transcription, and translation in tumors after treatment. (H) The impacts of the formulations on tumor proliferation and apoptosis, as measured by Ki67 (top) and TUNEL (bottom) staining. ∗ p < 0.05, considered as statistically significant; ∗∗∗∗ p < 0.0001, each bar chart represents the mean ± SD.

Article Snippet: BstZ17I-HF endonuclease (R3594L), rCutSmart Buffer (B6004S), and HiScribe T7 ARCA mRNA in vitro transcription kit (E2060S) were purchased from NEB (MA, USA).

Techniques: Expressing, TUNEL Assay, Staining

Journal: Nucleic Acids Research

Article Title: Toehold-VISTA: a machine learning approach to decipher programmable RNA sensor-target interactions

doi: 10.1093/nar/gkag097

Figure Lengend Snippet: Generalization of toehold-VISTA across reporters and expression contexts. ( A ) Schematic of VISTA-designed toehold switches using a LacZ reporter for colorimetric output. ( B ) Endpoint photographs of cell-free transcription-translation reactions showing colorimetric responses for truncated, full-length, and non-cognate SARS-CoV-2 N gene targets. ( C ) Time-course absorbance measurements (570 nm) demonstrating activation kinetics for truncated and full-length targets relative to non-cognate controls. Reaction rate was determined at 60- and 70-min timepoints. Quantification of OFF-state ( D ) and ON-state absorbance for truncated ( E ) and full-length ( F ) RNA targets comparing VISTA-ranked and tsgen2-ranked toehold switches. Fold-change in LacZ reaction rates for truncated ( G ) and full-length ( H ) targets, showing improved fold changes for VISTA-ranked switches. Comparisons made with two-tailed t -test. Bars represent mean and whiskers represent SD.

Article Snippet: In vitro cell-free protein synthesis system (New England Biolabs E6800L) solutions A (0.4) and B (0.3) were combined, with the remaining volume composed of 0.005 U RNase inhibitor (New England Biolabs M0314L), 0.6 mg ml −1 enzymatic substrate chlorophenol red-β-D-galactopyranoside (CPRG, Roche 10884308001 distributed by Sigma), linear DNA templates comprising target or sensor-LacZ, and ultrapure DNase/RNase-free DI water to a total volume of 5 μl.

Techniques: Expressing, Activation Assay, Two Tailed Test